Normal Frog Ringer (NFR) is used for lab experiments with Xenopus laevis oocytes to guarantee a physiological environment with respect to homeostasis, pH and osmolarity. Product information 10x concentrate NFR solution autoclaved Instructions for use: 10x concentrate: For 1000 ml NFR Solution: Pour 100 ml NFR (10x) into a flask Add deionized water to a final volume of 1000 ml Adjust to pH 7.4 Use immediately once opened. Store at 4 - 8 °C Ional composition of NFR Solution NaCl = 90 mM KCl = 2 mM CaCl2 = 2 mM MgCl2 = 1 mM HEPES = 5 mM
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